【專利核准公告】P101018A-乙基化胸腺嘧啶DNA加成產物之偵測方法「METHOD OF DETECTING ETHYLATED THYMIDINE DNA ADDUCTS」(已終止維護)
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專利國別 |
美國 |
發表日期 |
2014-10-08 09:54:10 |
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本校案號 |
P101018A |
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專利名稱 |
乙基化胸腺嘧啶DNA加成產物之偵測方法「METHOD OF DETECTING ETHYLATED THYMIDINE DNA ADDUCTS」 |
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發明人 |
陳皓君、王薏菁、林文棚 |
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所屬院 |
理學院 |
提案單位 |
化學暨生物化學系所
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類型 |
發明 |
專利權人 |
國立中正大學 |
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申請號 |
13/664,020 |
申請日期 |
2012-10-30 |
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公開號 |
US 2013-0295558 A1 |
公開日期 |
2013-11-07 |
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證書號 |
US 8,741,562 B2 |
核准日期 |
2014-06-03 |
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國際分類號 |
C12Q-001/68(2006.01) |
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專利權期間 |
2012.10.30~2032.10.30(延長84天至2033.01.22) |
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專利摘要 |
A method of analyzing ethylated thymidine DNA adducts is disclosed. The method comprises the steps of: providing leukocyte DNA; adding at least one isotope-labeled internal standard and a plurality of enzymes to the leukocyte DNA, and hydrolyzing the leukocyte DNA into a plurality of nucleosides; using a solid-phase extraction column to extract the plurality of nucleosides; and using a stable isotope dilution nanoflow liquid chromatography-nanospray ionization tandem mass spectrometry to detect and quantify at least one ethylated thymidine DNA adduct in the plurality of extracted nucleosides. |
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